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Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Target Species
Mouse
Host Species
Goat
Conjugate
Biotin
Applications
Western Blot
Isotype
IgG
Concentration
LYOPH
Antigen
IgG
Gene Symbols
IGHG1
Regulatory Status
RUO
Purification Method
Affinity Purified
Dilution
Western Blot
Gene Alias
Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez)
3500
Immunogen
Mouse IgG
Classification
Polyclonal
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Primary or Secondary
Secondary
Test Specificity
Detects mouse IgG 1 IgG 2A IgG 2B IgG 3 heavy and light chains. In direct ELISAs, approximately 40% cross-reactivity with rat IgG, and less than 5% cross-reactivity with human IgG and rabbit IgG is observed.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Target Species
Goat
Host Species
Rabbit
Conjugate
Biotin
Applications
Western Blot
Isotype
IgG
Concentration
LYOPH
Antigen
IgG
Gene Symbols
IGHG1
Regulatory Status
RUO
Purification Method
Affinity Purified
Dilution
Western Blot
Gene Alias
Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez)
3500
Immunogen
Goat IgG
Classification
Polyclonal
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Primary or Secondary
Secondary
Test Specificity
Detects goat IgG. In Western blot, less than 5% cross-reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.